percp cyanine5 5 anti mouse cd8 Search Results


percp cyanine 5.5-labelled anti-mouse cd8  (Becton Dickinson)
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Becton Dickinson percp cyanine 5.5-labelled anti-mouse cd8
( A ) Thymocytes or peripheral CD4 + T cells were isolated from spleens of littermate or Atg5 f/f dLck-cre mice. Cells were stimulated when indicated by 50 ng/mL PMA and 1 µg/mL Ionomycin for 18 hours. During the last 4 hours of stimulation, cells were treated when indicated with pepstatin A and E64d. Cell lysates were then processed by SDS-PAGE and blotted against ATG5 and LC3. Representative experiment of at least three replicates. Complete images of different blotting of the same membrane, including data with wild type C57BL/6 (B6) mice, are shown in Figure . ( B ) Left: Representative dot plots of thymocytes stained by anti-CD4 and <t>anti-CD8</t> Abs (left) from B6, littermate (LM), Atg5 f/f dLck-cre mice. Right: proportions among thymocytes of double negative (DN), CD4 single positive (CD4 SP), CD8 single positive (CD8 SP) or double positive (DP) cells (n = 5 LM and n = 4 Atg5 f/f dLck-cre mice) ( C ). Representative dot plots of spleen cell staining by anti-CD4 and anti-CD8 Abs, after gating on TCRβ + B220 − cells from B6, littermate LM, Atg5 f/f dLck cre mice. ( D ) Percentages of CD4 + , CD8 + T cells, and B cells among spleen cells (n = 13 B6, n = 17 LM and n = 17 Atg5 f/f dLck-cre mice). ( E ) Absolute numbers of naive, effector memory (EM, CD44 hi CD62 lo ) and central memory (CM, CD44 hi CD62L hi ) CD4 + and CD8 + T cells in spleens (n = 12 for each genotype) among TCR-β + -gated cells. Each point represents the value obtained with one mouse. LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. *p < 0.05, ***p < 0.001.
Percp Cyanine 5.5 Labelled Anti Mouse Cd8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percp cyanine 5.5-labelled anti-mouse cd8 - by Bioz Stars, 2026-08
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( A ) Thymocytes or peripheral CD4 + T cells were isolated from spleens of littermate or Atg5 f/f dLck-cre mice. Cells were stimulated when indicated by 50 ng/mL PMA and 1 µg/mL Ionomycin for 18 hours. During the last 4 hours of stimulation, cells were treated when indicated with pepstatin A and E64d. Cell lysates were then processed by SDS-PAGE and blotted against ATG5 and LC3. Representative experiment of at least three replicates. Complete images of different blotting of the same membrane, including data with wild type C57BL/6 (B6) mice, are shown in Figure . ( B ) Left: Representative dot plots of thymocytes stained by anti-CD4 and anti-CD8 Abs (left) from B6, littermate (LM), Atg5 f/f dLck-cre mice. Right: proportions among thymocytes of double negative (DN), CD4 single positive (CD4 SP), CD8 single positive (CD8 SP) or double positive (DP) cells (n = 5 LM and n = 4 Atg5 f/f dLck-cre mice) ( C ). Representative dot plots of spleen cell staining by anti-CD4 and anti-CD8 Abs, after gating on TCRβ + B220 − cells from B6, littermate LM, Atg5 f/f dLck cre mice. ( D ) Percentages of CD4 + , CD8 + T cells, and B cells among spleen cells (n = 13 B6, n = 17 LM and n = 17 Atg5 f/f dLck-cre mice). ( E ) Absolute numbers of naive, effector memory (EM, CD44 hi CD62 lo ) and central memory (CM, CD44 hi CD62L hi ) CD4 + and CD8 + T cells in spleens (n = 12 for each genotype) among TCR-β + -gated cells. Each point represents the value obtained with one mouse. LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. *p < 0.05, ***p < 0.001.

Journal: Scientific Reports

Article Title: CD4 T cell autophagy is integral to memory maintenance

doi: 10.1038/s41598-018-23993-0

Figure Lengend Snippet: ( A ) Thymocytes or peripheral CD4 + T cells were isolated from spleens of littermate or Atg5 f/f dLck-cre mice. Cells were stimulated when indicated by 50 ng/mL PMA and 1 µg/mL Ionomycin for 18 hours. During the last 4 hours of stimulation, cells were treated when indicated with pepstatin A and E64d. Cell lysates were then processed by SDS-PAGE and blotted against ATG5 and LC3. Representative experiment of at least three replicates. Complete images of different blotting of the same membrane, including data with wild type C57BL/6 (B6) mice, are shown in Figure . ( B ) Left: Representative dot plots of thymocytes stained by anti-CD4 and anti-CD8 Abs (left) from B6, littermate (LM), Atg5 f/f dLck-cre mice. Right: proportions among thymocytes of double negative (DN), CD4 single positive (CD4 SP), CD8 single positive (CD8 SP) or double positive (DP) cells (n = 5 LM and n = 4 Atg5 f/f dLck-cre mice) ( C ). Representative dot plots of spleen cell staining by anti-CD4 and anti-CD8 Abs, after gating on TCRβ + B220 − cells from B6, littermate LM, Atg5 f/f dLck cre mice. ( D ) Percentages of CD4 + , CD8 + T cells, and B cells among spleen cells (n = 13 B6, n = 17 LM and n = 17 Atg5 f/f dLck-cre mice). ( E ) Absolute numbers of naive, effector memory (EM, CD44 hi CD62 lo ) and central memory (CM, CD44 hi CD62L hi ) CD4 + and CD8 + T cells in spleens (n = 12 for each genotype) among TCR-β + -gated cells. Each point represents the value obtained with one mouse. LM and Atg5 f/f dLck-cre mice are compared by Mann Whitney U Test. *p < 0.05, ***p < 0.001.

Article Snippet: For surface stainings: allophycocyanin (APC)-cyanine 7-labelled anti-mouse TCR-β (clone H57-597, BD Biosciences 553139), phycoerythrin (PE)-labelled anti-mouse CD62L (clone MEL-14, BD Biosciences 553151), fluorescein isothiocyanate (FITC), APC or peridinin chlorophyll (PerCP) cyanine 5.5-labelled anti-mouse B220 (clone RA3-6B2, BD Biosciences, 553087, 553092, and 552771), FITC-labelled anti-mouse CD3ε (clone 145-2C11, BD Biosciences 553061), APC cyanine7, PE or PerCP cyanine 5.5, APC-labelled anti-mouse CD4 (clone GK1.5,552051, eBioscience 12-0041 or clone RM4-5, BD Biosciences, 550954, 553051), PerCP cyanine 5.5-labelled anti-mouse CD8 (clone 53-6.7, BD Biosciences, 551162), APC cyanine 7 or FITC-labelled anti-mouse CD44 (clone IM7, BD Biosciences 559250, 103027, 553133), PE-labelled CD127 (IL-7R, clone A7R43 eBioscience 12-1271-82).

Techniques: Isolation, SDS Page, Staining, MANN-WHITNEY

( A ) Percentages of dead cells measured by Annexin V staining ex vivo from the spleen for the following populations: effector memory (EM, CD44 hi CD62 lo ) and central memory (CM, CD44 hi CD62L hi ) after gating on TCRβ + CD4 + cells or TCRβ + CD8 + cells, from littermate (LM) and Atg5 f/f dLck-cre mice (n = 8 for each gentotype). ( B ) Percentages of viable cells defined as Annexin V − /propidium iodide (PI) − , defined after stimulation of purified CD4 + (left) or CD8 + T cells (right) under the indicated conditions (non-stimulated, stimulated by anti-CD3, by anti-CD3 and anti-CD28, or by 50 ng/mL PMA and 1 µM ionomycin, n = 4 for each genotype). ( C ) Staining of CD4 + or CD8 + T cells purified from LM or Atg5 f/f dLck-cre mice by mitotracker deep red and mitotracker green. Gates could be defined for cells with either high load of mitochondria (mitotracker deep red hi , mitotracker green hi ), low mitochondrial content (mitotracker deep red low , mitotracker green low ), or with damaged mitochondria (mitotracker green hi , mitotracker deep red low ). On the right, histograms show the results of independent experiments (n = 4 for each genotype). ( D ) Ex vivo staining of spleen cells from LM or Atg5 f/f dLck-cre mice, by Mitosox, after gating on TCRβ + CD4 + cells or TCRβ + CD8 + . (Top) representative histograms are shown and (bottom) a summary of different experiments is shown. LM and Atg5 f/f dLck-cre mice are represented and compared by Mann Whitney U Test. **p < 0.01, *p < 0.05.

Journal: Scientific Reports

Article Title: CD4 T cell autophagy is integral to memory maintenance

doi: 10.1038/s41598-018-23993-0

Figure Lengend Snippet: ( A ) Percentages of dead cells measured by Annexin V staining ex vivo from the spleen for the following populations: effector memory (EM, CD44 hi CD62 lo ) and central memory (CM, CD44 hi CD62L hi ) after gating on TCRβ + CD4 + cells or TCRβ + CD8 + cells, from littermate (LM) and Atg5 f/f dLck-cre mice (n = 8 for each gentotype). ( B ) Percentages of viable cells defined as Annexin V − /propidium iodide (PI) − , defined after stimulation of purified CD4 + (left) or CD8 + T cells (right) under the indicated conditions (non-stimulated, stimulated by anti-CD3, by anti-CD3 and anti-CD28, or by 50 ng/mL PMA and 1 µM ionomycin, n = 4 for each genotype). ( C ) Staining of CD4 + or CD8 + T cells purified from LM or Atg5 f/f dLck-cre mice by mitotracker deep red and mitotracker green. Gates could be defined for cells with either high load of mitochondria (mitotracker deep red hi , mitotracker green hi ), low mitochondrial content (mitotracker deep red low , mitotracker green low ), or with damaged mitochondria (mitotracker green hi , mitotracker deep red low ). On the right, histograms show the results of independent experiments (n = 4 for each genotype). ( D ) Ex vivo staining of spleen cells from LM or Atg5 f/f dLck-cre mice, by Mitosox, after gating on TCRβ + CD4 + cells or TCRβ + CD8 + . (Top) representative histograms are shown and (bottom) a summary of different experiments is shown. LM and Atg5 f/f dLck-cre mice are represented and compared by Mann Whitney U Test. **p < 0.01, *p < 0.05.

Article Snippet: For surface stainings: allophycocyanin (APC)-cyanine 7-labelled anti-mouse TCR-β (clone H57-597, BD Biosciences 553139), phycoerythrin (PE)-labelled anti-mouse CD62L (clone MEL-14, BD Biosciences 553151), fluorescein isothiocyanate (FITC), APC or peridinin chlorophyll (PerCP) cyanine 5.5-labelled anti-mouse B220 (clone RA3-6B2, BD Biosciences, 553087, 553092, and 552771), FITC-labelled anti-mouse CD3ε (clone 145-2C11, BD Biosciences 553061), APC cyanine7, PE or PerCP cyanine 5.5, APC-labelled anti-mouse CD4 (clone GK1.5,552051, eBioscience 12-0041 or clone RM4-5, BD Biosciences, 550954, 553051), PerCP cyanine 5.5-labelled anti-mouse CD8 (clone 53-6.7, BD Biosciences, 551162), APC cyanine 7 or FITC-labelled anti-mouse CD44 (clone IM7, BD Biosciences 559250, 103027, 553133), PE-labelled CD127 (IL-7R, clone A7R43 eBioscience 12-1271-82).

Techniques: Staining, Ex Vivo, Purification, MANN-WHITNEY